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Francelab is offering a wide range of sera, cell culture media for Research and Development and Quality Control laboratories in the field of Lifesciences and Biopharmaceuticals.

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Cell Culture Media

Mc Coy's media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media support the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Basal Media Eagle is principaly used for diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts.

These media were formulated as a reduced serum supplemented medium for the cultivation of human microvascular endothelials cells. For other microvascular cells it is recommended to use it with growth factors like EGF or hormons like hydorcortisone.

Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

A long term cultivation of cells required addition of a serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Dulbecco's Modified Eagle Medium (DMEM) is for supporting and maintening a broad spectrum of mamamalian cell types.

Ham's F14 medium was developed from the Ham's F12 which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells.
Ham's F14 contains a double concentration of amino acids compared to the Ham's F12. This product is also supplemented in Calcium chloride and Ascorbic Acid.

Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO2 gas.

Fisher's medium supports the growth and maintenance of Murine Leukemic cells.

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium.

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