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Francelab is offering a wide range of sera, cell culture media for Research and Development and Quality Control laboratories in the field of Lifesciences and Biopharmaceuticals.

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Cell Culture Media

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium.

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukemic cells.

GMEM was originally formulated for BHK 21 cells growing. It is intended for use with adherent cell lines.

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO2. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Schneider's Insect medium was developed to support the growth of excised imaginal disks from the fruitfly, Drosophila melanogaster. When supplemented with 5-20% heat-inactivated fetal bovine serum, Schneider's medium has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

Ham's Medium were originally developed for the clonal growth of Chinese Hamster Ovary (CHO) cells, HeLa cells and mouse L-cells, with or without serum supplementation depending the cells type. Ham's F10 is a medium of choice for supporting the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. It is a popular medium for growth of fastidious cell lines.

Mc Coy's media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media support the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Basal Media Eagle is principaly used for diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts.

These media were formulated as a reduced serum supplemented medium for the cultivation of human microvascular endothelials cells. For other microvascular cells it is recommended to use it with growth factors like EGF or hormons like hydorcortisone.

Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

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